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Followed by cDNA synthesis using the High-Capacity cDNA Reverse Transcription Kit (Bio-Rad, Hercules, CA)

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coli , except for S49C, which is an already described cysteine mutant used for site-specific labeling of SpyCatcher 21

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Thus, being able to measure all forms of GSH in one well significantly reduced the sample volume required for our experiment and mitigated inter-plate variation as a confound in data analysis

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