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GSTs function by conjugating reduced GSH and catalyzing the attack on foreign compounds or oxidative stress products, generally forming less-reactive materials that can be readily excreted
Western blot assay K7M2 cells were incubated with the PBS, Ru(II)-OH, NP2, or NP4 (10 M Ru) for 12 h at 37 C, and the supernatant was subsequently co-incubated with RAW264.7 cells
Chemosphere 58:11531159 Paetzold SC, Ross NW, Richards RC, Jones M, Hellou J, Bard SM (2009) Up-regulation of hepatic ABCC2, ABCG2, CYP1A1, and GST in multixenobiotic-resistant killifish ( Fundulus heteroclitus ) from the Sydney Tar Ponds, Nova Scotia, Canada
SDS-PAGE gel electrophoresis was performed, equal amounts of protein (40 g) were separated on 10% gels using SDS-PAGE and the samples were then transferred to polyvinylidene fluoride membranes
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