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The FDA has framed the letters as part of a broader crackdown on direct-to-consumer drug advertising from the Department of Health and Human Services, which also included dozens of letters to drugmakers about promotional activities

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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h

1 Recently, GLP-1s have emerged as alternatives due to their effects on weight reduction and glycemic control