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The supernatant was collected from centrifugation at 15,000 g for 20 min at 4 C and protein was quantified with Bradford reagent

If dual agonists produce faster or more noticeable results, patients may maintain adherence better compared to slower-acting single agonists

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The addition of GSH or expression of Grxs 1 and 2 significantly improves mutant SOD1 solubility in cell culture, whereas reducing the levels of intracellular GSH decreases SOD1 solubility, suggesting that GSH reduction promotes the aggregation of mutant SOD1 (Guareschi et al., 2012)