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The suspension was freeze-thawed 3x at 80C, sonicated 3x 15 s then spun at 12 k RPM for 30 min to pellet the insoluble material

doi: 10.1016/S0140-6736(08)60725-4 62 HabermanRHMacFarlaneKACatronSSamuelsJBlankRBToproverMet al

A narrative review of the potential pharmacological influence and safety of ibuprofen on coronavirus disease 19 (COVID-19), ACE2, and the immune system: a dichotomy of expectation and reality