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Weeks 23: Weight loss becomes measurable on a scale for many patients, typically ranging from 13 pounds per week depending on diet adherence and baseline metabolism

DNA methylation Genomic DNA was isolated from the liver tissue or cultured cells using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen, Germantown, MD) following the manufacturers instructions

The American Diabetes Association Standards of Care emphasizes the importance of resistance training and adequate protein intake during pharmacologic weight loss to maintain muscle mass, and creatine supplementation may support these goals

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